.. ######################## .. _format-fastq-files: .. ######################## Sample name details =================== ``XICRA`` accepts a wide range of files names. Format for fastq files can be: name.fastq.gz, name_1.fastq.gz, name_R2.fastq.gz, name_L001_R1.fastq.gz, etc. Here we provide some guidelines of the format. Length limitation ----------------- There is a limitation for the sample ID ('name') of 10 characters. ** XICRA provides an option to rename samples if necessary: module prep option --rename ** Single end files ---------------- It is possible to provide NGS single-end files although some steps of the process could be accomplished using single-end files. ** Use option --single-end in the different XICRA modules ** name.fastq.gz name.fastq name.fq Paired-end files ---------------- Paired-end files are full supported. The format for these files are: name_1.fastq.gz or name_R1.fastq.gz name_2.fastq.gz or name_R2.fastq.gz Lane information: ----------------- In some cases, files might contain lane information (*L00x* and/or *00x*). ``XICRA`` supports these names as long as follow these examples: name_L00x_R1.fastq.gz name_L00x_R2.fastq.gz name_L00x_1.fastq.gz name_L00x_2.fastq.gz name_L00x_R1_00x.fastq.gz name_L00x_R2_00x.fastq.gz ** If you need to merge fastq files from different lanes, use option --merge within module prep ** Extensions: ----------- name_L00x_R2.fastq name_L00x_R2.fq name_L00x_R2.fastq.gz name_L00x_R2.fq.gz