Software Information

This is a general guide for the software that we employed along the XICRA pipeline.

FastQC

FastQC [1] is a quality control tool for high throughput sequence data. It aims to provide a simple way to do some quality control checks on raw sequence data coming from high throughput sequencing pipelines. It provides a modular set of analysis which you can use to give a quick impression of whether your data has any problems of which you should be aware before doing any further analysis.

The main functions of FastQC are

  • Import of data from BAM, SAM or FastQ files (any variant)

  • Providing a quick overview to tell you in which areas there may be problems

  • Summary graphs and tables to quickly assess your data

  • Export of results to an HTML based permanent report

  • Offline operation to allow automated generation of reports without running the interactive application

Read further information about FastQC: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/

Cutadapt

STAR

Feature counts

mirTop

Miraligner

MINTmap

sRNAbench

Optimir